antimyc antibodies (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc antimyc antibodies
Antimyc Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antimyc+antibodies/pm40898765-151-14-17
Average 86 stars, based on 1 article reviews

antimyc antibodies - by Bioz Stars, 2026-09

86/100 stars

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Chromatin Immunoprecipitation:

Article Title: Broadly active zinc finger protein-guided transcriptional activation of HIV-1
Article Snippet: .. ChIP analysis was carried out on the transfected pMo-HEK cells for the myc-tagged activating complexes using antimyc antibodies (Cell Signaling Technology, Danvers, MA, USA). .. The ChIP protocol was performed 72 h post-transfection using previously described protocols.10,59,60 The ChIP elutes were purified using the MinElute PCR purification kit (QIAGEN, Hidlen, Germany) and analyzed by qPCR.

Transfection:

Article Title: Endogenous thrombopoietin promotes non-small-cell lung carcinoma cell proliferation and migration by regulating EGFR signalling.
Article Snippet: The selected bands were sent to Shanghai Applied Protein Technology for mass spectrometric analysis after Coomassie blue staining. .. Assays were performed as described previously.28 To verify interaction between transfected TPO and EGFR, A549 and H1299 cells were collected 48 h after transfection with pCMV6-Myc-DDK-TPO plasmids, and then, cell lysates were immunoprecipitated with antiMyc antibodies (# 2276; Cell Signaling Technology) or control IgG mouse antibodies. .. To verify interaction between endogenous TPO and EGFR, cell lysates were immunoprecipitated with anti-TPO antibodies (sc-374045; Santa Cruz Biotechnology) or control IgG mouse antibodies.

Incubation:

Article Title: PCDHGB7 inhibits the progression of triple-negative breast cancer by suppressing XRCC5/MYC-mediated ribosome biogenesis.
Article Snippet: .. These protein samples were subsequently incubated with anti-flag (specific to PCDHGB7, #20543-1-AP, Proteintech), or antiMYC antibodies (#18583S, Cell Signalling), or a control IgG (#30000-0-AP, Proteintech) for 24 h. To facilitate immunoprecipitation, protein A/G-agarose beads were introduced to the mixture and allowed to incubate for an additional 24 h. The immunocomplexes captured on the beads were then resolved by SDS-PAGE, followed by immunoblot analysis using the appropriate antibodies to detect the proteins of interest. .. The cells underwent treatment with cycloheximide (.1 mg/mL, Sigma-Aldrich) for 10 min at a temperature of 37◦C.

Control:

Immunoprecipitation:

Western Blot:

Flow Cytometry:

Article Title: Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells.
Article Snippet: .. Antimyc antibodies (Cell Signaling Technologies #2233S, #3739S, and #2279S) were used at a 1:100 ratio while antiV5 (ThermoFisher Scientific #12-679642) and antiFLAG (R&D Systems #IC8529G-100) antibodies were used at a 1:50 ratio for flow cytometry assays. ..

Article Title: Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells
Article Snippet: .. Antimyc antibodies (Cell Signaling Technologies #2233S, #3739S, and #2279S) were used at a 1:100 ratio while antiV5 (ThermoFisher Scientific #12-679642) and antiFLAG (R&D Systems #IC8529G-100) antibodies were used at a 1:50 ratio for flow cytometry assays. ..

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